BRCA1 contains at least two nuclear localization signals and is proposed to be a tumor suppressor protein.
It is a serine phosphoprotein that undergoes hyper-phosphorylation during late G1-and S-phases of the cell cycle and is transiently dephosphorylated early after M-phase.1 BRCA1 protein alters in a qualitative and quantitative manner during cell cycle progression.1 The amount of BRCA1 protein is highest during S-phase and remains elevated toward G2/M, before it declines in early G1-phase.1 Inherited loss of BRCA1 function confers an increased susceptibility for both breast and ovarian cancer.
Applications:Suitable for use in Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunofluorescence:1:100-1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human BRCA1 when phosphorylated at serine 988.
Isotype：IgG
Calc Applications Abbrev：IF
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to human BRCA1 around the phosphorylation site of serine 988 (I-K-SP-F-V).
