Bim/BOD is a group of three splice variants, BimEL, BimL and BimS, with apparent molecular masses of ~33, 25, and 15kD, respectively.
Bim is a critical regulator of lymphocyte homeostasis, acts as a barrier against autoimmune disease and is necessary for the apoptotic response to certain stress signals.
All isoforms of Bim contain the C-terminal Bcl-2 homology domain known as BH3.
This domain is found in all apoptotic proteins in the Bcl-2 family that includes Bad, Bax, Bid, Bik, Hrk and Bim/BOD.
Of the three isoforms, BimS is the most potent inducer of apoptosis.
The three isoforms bind an 8kD dynein light chain, LC8 (also known as PIN or Dlc-1), with mediates the function of BimEL and BimL.
BimEL and BimL are sequestered to this microtubular dynein motor complex by binding LC8 in the cytosol.
Certain apoptotic stimuli release Bim from the complex which allows it to translocate to the mitochondria and bind to Bcl-2 or its homologues, thereby promoting apoptosis.
Bim is downregulated by either of two Ras pathways (Raf/MAPK or PI3-K/mTOR).
Downregulation is a critical step in the maintenance of hematopoietic cell survival while activation of Bim is a primary trigger for negative selection of autoreactive T cells during their development in the thymus.
In Bim deficient mice homeostasis of the lymphoid system was disrupted although no other organs appeared to be affected.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1,000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse Bim. Species Cross-Reactivity: human, rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic non-phosphopeptide corresponding to mouse BIM around the phosphorylation site of serine 65 (P-A-SP-P-G).
