84-1167-73　［取扱停止］Bad (Bcl2 Antagonist of Cell Death, Bcl-2-binding Component 6, Bcl-2-like Protein 8, Bcl2-L-8, Bcl-XL/Bcl-2-associated Death Promoter, BBC6, BCL2L8) 50ug　B0003-52

特徴

• BAD is a ~23kD member of the Bcl-2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl-2 and Bcl-xL, thus preventing them from binding with BAX.
• BAD is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, it translocates to the cytoplasm.
• The phosphorylation status of BAD represents a key checkpoint for death or cell survival.
• BAD is phosphorylated on serine 128 by cdc2 and c-Jun N-terminal kinase (JNK).
• JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of BAD by opposing the inhibitory effect of growth factor on BAD-mediated apoptosis.
• Therefore, linking stress–activated pathway to cell death machinery in neurons.
• Cdc2-induced phosphorylation of BAD serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins.
• The latter interaction is critical for BAD phosphorylation at serine 155, a site within the BH3 domain that leads to the release of Bcl-xL and the promotion of cell survival.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1,000Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.