84-1167-63　［取扱停止］Aquaporin 4 (AQP4, Aquaporin-4, AQP-4, WCH4, MIWC, Mercury-insensitive Water Channel) 100ug　A3000-14C

特徴

• Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells.
• The gene of AQP4 is mapped to 18q11.2-q12.1.
• Similar to other aquaporins, the AQP4 gene is composed of 4 exons encoding 127, 55, 27, and 92aa separated by introns of 0.8, 0.3, and 5.2kb.
• Unlike other aquaporins, an alternative coding initiation sequence (designated exon 0) was located 2.7kb upstream of exon 1.
• When spliced together, M1 and the subsequent 10aa are encoded by exon 0; the next 11aa and M23 are encoded by exon 1.
• AQP4 is expressed in astrocytes and is upregulated by direct insult to the central nervous system.
• AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis.
• It is abundant in mammalian brain and is concentrated in astrocytic foot processes at the blood-brain barrier.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.