AMPK1 belongs to the ser/thr protein kinase family.
It is the catalytic subunit of the 5'-prime- AMP-activated protein kinase (AMPK).
AMPK is a cellular energy sensor conserved in all eukaryotic cells.
The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio.
AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation.
It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.
Applications:Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:200Western Blot: 1:500-1:1,000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human AMPK1. Species Crossreactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic non-phosphopeptide corresponding to human AMPK1 around the phosphorylation site of threonine 174 (L-R-TP-S-C).
