84-1167-41　［取扱停止］AMP Activated Protein Kinase beta1, phosphorylated (Ser182) (5'-AMP-activated Protein Kinase Subunit beta-1, AMPK Subunit beta-1, AMPKb, PRKAB1, AMPK) 50ug　A1475-03L

特徴

• AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals, and plays a key role in regulation of energy homeostasis.
• The kinase is activated by an elevated AMP-ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia and ischemia.
• AMPK is also involved in apoptosis.
• AMP plays an essential role in the activation of AMPK.
• Firstly, it is required for AMPK phosphorylation by the upstream kinase AMPKK at Thr172.
• AMPK phosphorylation by AMPKK raises AMPK activity at least 50-fold.
• Secondly, AMP inhibits the dephosphorylation and inactivation of AMPK by PP2C.
• The beta1 subunit is post-translationally modified by myristoylation and multi-site phosphorylation.
• Phosphorylation at serine 108 of the beta1 subunit seems to be required for the activation of AMPK enzyme.
• Phosphorylation at serines 24, 25 and 182 of the beta1 subunit affects its cellular localization.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:4,000 Western Blot: 1:500-1:1,000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.