Alpha-2-adrenergic receptor (ADRA2), also known as platelet type adrenoceptor alpha-2A, is a member of G protein-coupled receptor superfamily.
This gene, which can identify a DraI RFLP of the ADRAR gene, mapped to 10q23-q25, is found in the distal region of mouse chromosome 19 and abundantly expressed in giant cell.
ADRA2 acts a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system.
It has 3 highly homologous subtypes: ADRA2A; ADRA2B and ADRA2C.
Studies in mouse suggested that both the ADRA2A and ADRA2C subtypes are required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons.
ADRA2A receptors inhibited transmitter release at high stimulation frequencies, whereas the ADRA2C subtype modulated neurotransmission at lower levels of nerve activity.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml Immunohistochemistry (paraffin): 1-2ug/ml, boiling the sections is required
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH20.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.
Specificity：Recognizes human ADRA2. Species Cross-Reactivity: mouse, rat, rabbit.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rb Rt
Immunogen：Synthetic peptide corresponding to a sequence mapping near the C-terminal of human ADRA2, identical to the related rat and mouse sequence.
