ABI1 is a human homolog of mouse Abl-interactor-1 (Abi1), mapped on 10p11.2.
ABI1 participate in the transduction of signals from Ras to Rac by regulating Rac-specific guanine nucleotide exchange factor (GEF) activities.
ABI1 dramatically promoted ABL1-mediated tyrosine phosphorylation of MENA, but not of other substrates.
Abi-1 regulates c-Abl-mediated phosphorylation of Mena by interacting with both proteins.
ABI1 plays a role in the leukemogenesis by translocating to MLL.
Applications:Suitable for use in Western Blot,, Immunohistochemistry and Immunofluorescence/Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/ml Immunohistochemistry (frozen/paraffin): 0.5-1ug/ml Immunofluorescence (IC): 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.
Specificity：Recognizes human ABI1. Species Cross-Reactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IC IF IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to a sequence at the C-terminal of human ABI-1. Species Sequence Homology: rat, and mouse different by one amino acid.
