14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins.
14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr.
Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein.
Expression of 14-3-3 gamma is higher in cells from vascular smooth muscle, skeletal and heart muscle.
In Alzheimer’s disease and Down syndrome level of 14-3-3 gamma in brain increases significantly.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot:1:500-1:1,000Immunohistochemistry:1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human 14-3-3 zeta. Species Cross-Reactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic non-phosphopeptide corresponding to human 14-3-3 zeta around the phosphorylation site of serine 58 (R-S-SP-W-R).
