Brorin (also VWC2, von Willebrand Factor C domain-containing protein 2) is a secreted, 48-49 kD member of the chordin family of proteins.
It is expressed by neurons in multiple sites, and serves to sequester BMP family proteins such as BMP-2 and BMP-6.
BMP antagonism is suggested to generate neurons from neural precursor cells at the expense of astrocytes.
Human Brorin precursor is 325aa in length.
It contains a 27aa signal sequence, one poly-Ala region (aa120-127), and two von Willebrand factor C domains (aa153-212 and 216-274) that likely serve as BMP interaction sites.
Over aa28-325, human Brorin shares 90% aa identity with mouse Brorin.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1ug/mlWestern Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human Brorin. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Brorin
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser28-Met325 from human VWC2, expressed in NSO cells
