TRIF (TIR domain-containing adaptor inducing IFN-b; also TICAM1) is a 105-110kD cytoplasmic adaptor molecule that mediates Toll receptor signaling.
It is widely expressed and associates with both TLR3 and TLR4.
Relative to TLR3, TRIF appears to activate IRF-3, -4, and -7, as well as NFkB and FADD.
Its action on FADD is through RIP1, and this induces apoptosis.
Human TRIF is 712aa in length and contains three TRAF6 bonding motifs (aa84-91, 248-255 and 299-309), one TIR domain (aa390-460), a Pro-rich region (aa614-678), and an overlapping RHIM domain (aa661-699).
The molecule is reported to form a homodimer.
There are multiple potential isoform variants.
One shows a 23aa substitution for aa31-162 accompanied by a Pro substitution for aa633-660, a second shows a 44aa substitution for aa352-712, and a third shows a 38aa substitution for aa271-712.
Over aa29-204, human TRIF shares 62% aa identity with mouse TRIF.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot:0.25ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um-filtered solution in PBS, 5% trehalose. Reconstitute with 100ul PBS, 0.02% sodium azide.
Specificity：Recognizes human TRIF. Species sequence homology: mouse (62%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Purified, E. coli-derived, recombinant human TIR domain-containing adaptor inducing IFN-b (aa29-204; Accession #Q8IUC6).
