TRIM21 (Tripartite motif-containing protein 21; also Ro(SS-A), 52kD Ro Protein/Ro52, and RING finger protein 81) is a 52-56kD member of the RING finger-B-box-coiled-coil family of proteins.
It is an E3 ligase that is found in both nucleus and cytoplasm, where it is often associated with microtubules.
TRIM21 ubiquitinates select proteins.
In B cells, it targets the Fc fragment of misfolded IgG, providing QC on its production.
In macrophages, it acts in a nondegradative manner on IRF8, promoting innate immunity.
Human TRIM21 is 475aa in length and contains one E3 ligase RING finger domain (aa16-55), a B-Box type zinc-finger region (aa92-123), a coiled-coil region (aa128-238) and a C-terminal PRY/B30.2 Ig binding domain (aa268-465).
TRIM21 is reported to form trimers.
Over aa195-293, human TRIM21 exhibits 72aa identity with mouse TRIM21.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 100ul PBS, 0.02% sodium azide.
Specificity：Recognizes human SSA1.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Purified, E. coli-derived, recombinant human TRIM21 (aa1-475; Accession # P19474).
