Proteinase 3 (PRTN3; also PR3/leukocyte, Myeloblastin and NP-4) is a 32-33kD member of the peptidase S1 family of enzymes.
It is expressed by monocytes and neutrophils, the latter of which either secretes it, sequesters it in azurophilic granules, or expresses it on the cell surface.
When secreted, it acts on HK and activates the kinin pathway.
In azurophilic granules, it aids in the digestion of phagocytosed material.
On the cell surface, it likely acts on ECM.
Human PRTN3 proprecursor is 231aa in length.
It contains an Ala26Glu27 propeptide that is removed during maturation, a 221aa mature enzyme (aa28-248), and an eight aa C-terminal propeptide (aa249-256).
Within the cell, a 35kD immature form exists; on the cell surface, both constitutively inactive, and induced active forms may be found, often in a noncovalent association with CD177/NB1.
Over aa26-249, human PRTN3 shares 68% aa identity with mouse PRTN3.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA:0.5-1ug/mlWestern Blot:1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS.
Specificity：Recognizes human PRTN3.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Purified, Sf 21-derived, recombinant human Proteinase 3 (aa26-249; Accession #P24158, with Val119Ile and Ser203Ala substitutions).
