Plexin A2 (also OCT) is a 220-230kD member of the plexin-A subfamily, plexin family of proteins.
It is found on cerebellar granule cells, sensory neurons, and cardiac neural crest cells.
It participates in cell migration and axon guidance, and does so by serving as a receptor for Sema6A and B, and as a coreceptor with neuropilin-1 for Sema3A and C.
Mature mouse Plexin A2 is an 1860aa type I transmembrane glycoprotein.
It contains a 1203aa extracellular domain (ECD) and a 636aa cytoplasmic region.
The ECD contains one Sema-domain (aa40-481), three PSI domains (aa500-845) and four IPT regions (aa848-1226).
There is one alternate start site 10aa upstream of the standard start site.
Over aa25-1227, mouse Plexin A2 is 96% aa identical to human Plexin A2.
Applications:Suitable for use in Flow Cytometry, ELISA, Immunocytochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml using bEnd.3 mouse endothelioma cell line and rat embryonic cortical neuron cellsFlow Cytometry 2.5ug/10e6 cells using bEnd.3 mouse endothelioma cell lineImmunocytochemistry: 10ug/ml using bEnd.3 mouse endothelioma cell line, HUVEC human endothelial cell and rat embryonic cortical neuron cellsOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse Plexin A2 extracellular domain. Species Crossreactivity: rat. <1% crossreactivity with rmPlexin A1, rmPlexin A3 and rhPlexin B1.
Isotype：IgG
Calc Applications Abbrev：E FC IC WB
Calc Crossreactivity：Mo Rt
Immunogen：Recombinant protein corresponding Met35-Pro1237 from mouse Plexin 2, expressed in CHO cells. Species Sequence Homology: human (96%).
