PLC-d3 (phospholipase C-delta 3) is an 105kD member of the d-type PLC isozyme family.
It is expressed in corneal epithelium, platelets, cardiac and skeletal muscle.
PLC-d3 preferentially hydrolyzes membrane PIP release, and DAG, activating PKC.
Human PLC-d3 is 789aa in length.
It contains a plextrin homology domain that tethers the enzyme to the membrane (aa63-172), three EF-hand motifs that spatially orient the molecule (aa182-285), a catalytic region (aa337-644), and a C2 domain that secures the catalytic region to its substrate (aa647-752).
There are multiple potential isoforms.
There is an alternate start site at Met54, a 40aa substitution for the C-terminal 248 aa, and a Leu substitution for aa102-109.
Over aa148-344, human PLC-d3 is 93% aa identical to mouse PLC-d3.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot:1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute in PBS, 0.02% sodium chloride.
Specificity：Recognizes human PLCD3. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Purified, E. coli-derived recombinant human PLC-d3 (aa148-344; Accession #Q8N3E9).
