84-1165-44　［受注停止］PLA2G16 (Group XVI Phospholipase A2, Adipose-specific Phospholipase A2, AdPLA, H-rev 107 Protein Homolog, HRAS-like Suppressor 3, HREV107, HREV107-3, Renal Carcinoma Antigen NY-REN-65, HRASLS3) 100ug　P4074-16

特徴

• PLA2G16 (Group 16 phospholipase A2; also HREV107 and AdPLA) is a Ca++-dependent 17-18kD member of the H-rev107 family of molecules.
• It is expressed intracellularly by white adipocytes, induced by insulin, and generates arachadonic acid from phospholipids.
• The arachadonic acid is converted to PGE2, which binds the EP3 receptor and decreases cAMP levels.
• This results in decreased hormone-sensitive lipase activity and increased adiposity.
• Human PLA216 is 16211aa in length.
• It is associated with both membranes and cytosol, and may possess a transmembrane segment between aa134-154.
• The enzymatic activity resides in His23 and Cys113.
• There is one potential isoform that shows a 20aa substitution for the first four amino acids.
• Over aa12-132, human PLA2G16 shares 88% aa identity with mouse PLA2G16.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA:0.5-1ug/mlWestern Blot:1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.