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- 特定メーカー試薬
- US Biological(antibodypab)
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84-1165-43 Osteopontin (2AR, Bone Sialoprotein 1, Calcium Oxalate Crystal Growth Inhibitor Protein, Early T-lymphocyte Activation 1 Protein, Minopontin, Secreted Phosphoprotein 1, SPP-1, Spp1, Eta-1, Op, Spp-1) (PE) 100Tests O8064-01N
84-1165-43 Osteopontin (2AR, Bone Sialoprotein 1, Calcium Oxalate Crystal Growth Inhibitor Protein, Early T-lymphocyte Activation 1 Protein, Minopontin, Secreted Phosphoprotein 1, SPP-1, Spp1, Eta-1, Op, Spp-1) (PE) 100Tests O8064-01N
特徴
- Osteopontin (OPN), is a secreted acidic phosphorylated glycoprotein originally isolated from bone matrix.
- It has been shown to bind to different cell types through interactions with various integrins and CD44 variants.
- It has important functions in bone metabolism and inflammatory processes.
- Applications:Suitable for use in Flow Cytometry.
- Other applications not tested.
- Recommended Dilutions:Flow Cytometry: 10ul labels10e6 cells.
- Validated on RAW264.7 cells.
- For intracellular staining, cells must first be fixed and permeabilized.
- Optimal dilutions to be determined by the researcher.
- Storage and Stability:Store product at 4°C in the dark.
- DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
- Dilute required amount only prior to immediate use.
- Further dilutions can be made in assay buffer.
- Caution: PE conjugates are sensitive to light.
- For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
- Size:100Tests
- Host:goat
- Source Antibody:mouse
- Grade:Affinity Purified
- Purity:Purified by immunoaffinity chromatography.
- Form:Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
- Specificity:Recognizes mouse Osteopontin. In sandwich ELISA, less than 3% crossreactivity with rhOPN is observed.
- Isotype:IgG
- Calc Applications Abbrev:FC
- Calc Crossreactivity:Mo
- Immunogen:Recombinant protein corresponding to Leu17-Asn294 (Glu99Gly) from mouse Osteopontin, expressed in NS0 cells.
- Shelf Life:1year
- Swiss Prot Number:Q547B5
- EU Commodity Code:30021010
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