84-1165-41　NCOR1 (Nuclear Receptor Co-repressor 1, N-CoR, KIAA1047, N-CoR1, Nuclear Receptor Corepressor 1) 100ug　N0555-04F

特徴

• NCOR1 (Nuclear receptor Co-Repressor 1) is a 270kD member of the NCoR family of molecules.
• It is widely expressed, being found in hepatocytes, intestinal crypt cells, neural stem cells, plus immature thymocytes and erythrocytes.
• NCOR1 is a transcriptional repressor.
• It forms a complex with HDAC3, TAB2 and ZBTB33, and interacts with a ligand-independent THR:RXR heterodimer bound to select gene promoters.
• Human NCOR1 is 2440aa in length.
• It possesses one N-terminal repression domain (aa1-312), two DNA-binding SANT domains (aa437-674) and a second repression domain (aa737-1004).
• Multiple Ser, Thr and Tyr phosphorylation sites exist that regulate complex dissociation.
• There are multiple potential splice variants.
• Short poly Lys motifs serve as substitutions for the C-terminal 1900-1910aa.
• There is also a 16aa insertion after Glu727, coupled to either a Ile substitution for aa1842-1961, or a six aa substitution for aa31-145.
• Over aa1770-1947, human NCOR1 shares 96% aa identity with mouse NCOR1.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot:1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.