UNC13A (UNCoordinated 13A; also Munc13-1) is a 190-210kD member of the UNC-13 family, C2 domain protein superfamily of molecules.
It is expressed at synaptic junctions, particularly those involved in GABA, acetylcholine and glutamine transmission, and appears to be necessary for neurotransmitter release.
In the presynaptic region, UNC13A likely interacts with syntaxin 1, converting it from a closed to an open configuration, thus allowing for SNARE complex formation.
Human UNC13A is 1703aa in length.
It contains one C2 domain that mediates dimerization (aa1-79), a coiled-coil region (aa320-357), one DAG Zn-finger region (aa553-603), a second C2 domain (aa663-769), two MHD domains (aa1093-1512) and a third C2 domain (aa1532-1637).
Western blots of mouse UNC13A show 105kD and 70kD forms.
Over aa1525-1698, human UNC13A shares 98% aa identity with mouse UNC13A.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute in 500ul PBS
Specificity：Recognizes human Munc13-1. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to Asp1525-Gly1698 from human Munc13-1 expressed in E. coli.
