LRP-4 (Low density lipoprotein-related protein 4; also MEGF7 and LRP13) is a 215-225kD member of the LDLR family of proteins.
It is expressed on neurons, oocytes, spermatogonia and skeletal muscle cells, and binds multiple ligands, including WISE, apoE, MuSK and neuronal Agrin.
It serves to negatively regulate Wnt signaling during development, and to cluster AChRs at neuromuscular junctions.
Mature human LRP-4 is an 1885aa type I transmembrane glycoprotein.
It contains a 1705aa extracellular domain (ECD) (aa21-1725) plus a 159aa cytoplasmic region (aa1747-1905).
In the ECD, there are eight LDLR class A repeats (aa26-350), two EGF-like repeats (aa354-434) and 20 LDLR class B repeats that contain an intervening EGF-like domain (aa480-1610).
Over aa18-344, human LRP-4 shares 88% aa identity with mouse LRP-4.
Applications:Suitable for use in Direct ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml in human brain (cortex) tissue and rat cortical embryonic neuron/glial cellsImmunohistochemistry: 5-15ug/ml in immersion fixed paraffin-embedded sections of human brainstemOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human LRP-4. Species Crossreactivity: rat. In direct ELISA shows < 1% crossreactivity with recombinant human LRP-1, -1B, -5, -6 and recombinant human LDL R.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Rt
Immunogen：Recombinant protein corresponding to Gly18-Ser344 from human LRP-4, expressed in E. coli.
