LAT (Linker for activation of T cells; also pp36) is a 36-38kD type III (no signal sequence; extracellular N-terminus) transmembrane adaptor phosphoprotein.
It is expressed on mast cells, NK cells, monocytes (but not macrophages), platelets and T cells.
LAT is phosphorylated upon ligation of the TcR, GPVI, and FceRI, and serves as an anchor for several SH2-domain containing signaling proteins, including PLCg1, SLP76 and Grb2.
Human LAT is 262aa in length.
It contains a four aa extracellular segment with a 235aa cytoplasmic domain (aa28-262).
Palmitoylation occurs on Cys26 and 29.
There are 13 Ser/Thr phosphorylation sites plus five Tyr sites.
Multiple potential splice variants are reported.
There is an alternative start site 36aa upstream of the standard site that may be accompanied by a deletion of aa114-142.
There is also a deletion of aa88-97, and three substitutions: two aa for aa215-262, 38aa for aa55-262, and 50aa for aa35-262.
Over aa30-113, human LAT shares 73% aa identity with mouse LAT.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute in PBS, 0.02% sodium azide.
Specificity：Recognizes human LAT.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Purified, E. coli-derived recombinant human LAT (aa30-113; Accession #O43561).
