For intracellular staining, cells must first be fixed with 4% paraformaldehyde fixation buffer and permeabilized with 0.1% saponin balanced salt solution
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol:1.
Harvest cells and wash twice in saline buffer
2.
Cell surface staining may be done at this point following the manufacturer’s staining procedure
3.
Resuspend 5x10e5 cells in 0.5ml of cold 4% paraformldehyde fixation buffer and incubate at RT for 10 mins
4.
Following fixation, wash the cells twice in saline buffer, then once in 0.1% saponin saline buffer
5.
After permeabilization, add 10ul of I8443-21A and incubate the cells for 30 minutes at RT in the dark
6.
Wash the cells twice with 0.1% saponin saline buffer
7.
Resuspend the cells in saline buffer for final flow cytometric analysis.
As a control for this analysis, cells in a separate tube should be treated with APC-labeled sheep IgG antibody.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Specificity：Recognizes mouse IL22RA2.
Isotype：IgG
Calc Applications Abbrev：FC
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Thr19-Pro230 from mouse IL22RA2 expressed in NSO cells (Accession #Q80XF5 with D149E substitution).
