84-1165-33　Draxin (Dorsal Repulsive Axon Guidance Protein, Neucrin) 100ug　D9392-01

特徴

• Draxin (Dorsal repulsive axon guidance protein, neucrin) is a secreted, 58kD, presumable glycoprotein member of the Draxin family of molecules.
• In mammals, it is expressed in neurons (axons), astroglia, and likely cells of the developing somite.
• Draxin acts as a Wnt antagonist, apparently by binding to LRP6.
• The net effect is to block neural crest migration, and the organization of axons into functional tracts or bundles (fasciculation).
• Mature mouse Draxin is 318aa in length.
• It contains one potential N-linked glycosylation site, followed by a Cys-rich domain (aa268-327).
• Mature mouse Draxin (aa26-343) shares 80% aa identity with mature human Draxin, and 94% aa identity with mature rat Draxin.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Direct ELISA: 0.5-1ug/ml.
• The detection limit is ~0.5ng/well
• Western Blot:1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.