DOCK2 (Dedicator of cytokinesis protein 2) is a member of the DOCK-A family, DOCK180 superfamily of proteins.
Its predicted molecular weight is 210kD, although it may run approximately at 190kD in SDS-PAGE.
It is found in hematopoietic cell types, and regulates the activity of the GTPases Rac-1 and -2.
Human DOCK2 is 1830aa in length.
It contains one SH3 domain (aa8-69), a DHR-1 region that localizes DOCK2 to the plasma membrane (aa420-662), and a DHR-2 domain that binds to nucleotide-free GTPases and shows GEF activity (aa1115-1621).
There are two isoform variants, one that shows a 38aa substitution for Tyr1405, and a second that shows a start site at Met450 with accompanying deletions of aa462-494 and 630-766, and a 22aa substitution for aa934-1830.
Over aa420-642, human DOCK2 shares 96% aa identity to mouse DOCK2.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot:1g/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute in PBS, 0.02% sodium azide.
Specificity：Recognizes human DOCK2. Species sequence homology: mouse (96%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Gt Hu
Immunogen：Purified, E. coli-derived recombinant human DOCK2 (aa420-642; Accession #Q92608).
