CSRP2 (Cysteine-rich protein 2/CRP2; also Cysteine and Glycine-rich protein 2, LMO-5 and SmLIM) is a 21-22kD member of the CRP family of LIM domain-containing proteins.
It is expressed in arterial smooth muscle, hepatic stellate cells and fibroblasts.
CSRP2 is upregulated by TGF-b and downregulated by PDGF, and appears to interact with PIAS1 and CRP2-BP, thus promoting STAT signaling.
It also binds to SRF and GATA4 to initiate smooth muscle differentiation.
Human CSRP2 is 193aa in length and contains two 50aa LIM (for lin11/isl1/mec3) domains that contain two zinc-finger motifs (aa10-61 and 119-170), an NLS between aa64-69, and a Gly-rich C-terminus (aa176-187).
Full-length human CSRP2 shares 79% aa identity with human CSRP1.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot:2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute in PBS, 0.02% sodium azide.
Specificity：Recognizes human CSRP2.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Purified, E. coli-derived full-length recombinant human CSRP2 (aa1-193; Accession #Q16527).
