84-1165-29　CCNB2 (Cyclin B2, G2/Mitotic-specific Cyclin-B2) 100ug　C8453-28D

特徴

• Cyclin B2 (also CCNB2 and G2/mitotic-specific cyclin-B2) is a 45-46kD member of the Cyclin AB subfamily, cyclin family of proteins.
• It is widely expressed and associates with CDK1 providing substrate specificity to a phosphorylating complex.
• A phosphorCDK1:Cyclin B2 complex is associated with the Golgi apparatus, where it contributes to Golgi fragmentation during mitosis.
• It is also possible that Cyclin B2 can substitute for Cyclin B1 during the early stages of mitosis.
• Human Cyclin B2 is 398aa in length.
• It contains two cyclin box folds (aa201-290 and 298-383) and two substrate binding sites (aa165-254 and 264-347).
• Phosphorylation occurs at Ser92, Thr94, Ser99, Ser204, Ser392 and Ser398.
• Over aa1-101, human Cyclin B2 shares 79% aa identity with mouse Cyclin B2.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot:1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.