CDK4 (cyclin-dependent kinase 4) is a 34kD member of the CMGC Ser/Thr protein kinase family of enzymes.
Within the cell, pRb/retinoblastoma protein is normally unphosphorylated and suppresses cell growth at the G1 stage of the cell cycle.
When mitosis is required, CDK4 is phosphorylated, forms a complex with cyclin D1, 2 or 3, and phosphorylates pRb/retinoblastoma protein.
This inactivates pRb and cells initiate DNA synthesis.
Human CDK4 is 303aa in length.
It contains one protein kinase domain (aa6-295) and an activating phosphorylation site at Thr172.
There are three potential variants; one that shows an alternate start site at Met75, a second that shows a twoaa substitution for aa89-211, and a third that shows a 23aa substitution for aa89-303.
Full-length human and mouse CDK4 are 95% aa identical.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute in PBS, 0.02% sodium azide.
Specificity：Recognizes human CDK4. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Purified, E.coli-derived recombinant human CDK4. (aa1-303; Accession #P11802).
