Catalyzes the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as L-selectin ligands.
L-selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation.
Participates in biosynthesis of L-selectin ligand sialyl 6-sulfo Lewis X on L-selectin counter receptors CD43, GlyCAM-1 and MAdCAM-1.
Also involved in biosynthesis of L-selectin ligand recognized by MECA-79 antibody.
Plays a central role in lymphocyte trafficking during chronic inflammation.
Has a catalytic preference for core 2-branched mucin-type O-glycans.
Can use GlcNAcbeta1-6[Galbeta1-3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors.
Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP.
Its substrate specificity may be influenced by its subcellular location.
Applications:Suitable for use in ELISA, Neutralization and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1ug/mlWestern Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse CHST4.
Isotype：IgG
Calc Applications Abbrev：E N WB
Calc Crossreactivity：Mo
Immunogen：CHO-derived recombinant protein corresponding to Arg30-Gly388 of mouse Carbohydrate Sulfotransferase 4/CHST4.
