Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble.
Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton.
Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half.
The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding.
Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles.
Alternative splicing results in multiple transcript variants that encode the same isoform.
Applications:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilutions: Peptide ELISA Titer: 1:4000Western Blot: 0.1-0.3ug/ml ~85kD band observed in Human Peripheral Blood Mononucleocytes lysates (calculated MW of 61.3kD according to NP_003452.1).
The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human ZYX. Species sequence homology: canine. Reported variants represent identical protein: NP_003452.1, NP_001010972.1
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-QQREKPRVQEKQH, from the internal region of human YYX (NP_003452.1).
