This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance.
The encoded protein is one of two functional homologs to yeast Upf3p.
mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD).
When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons.
This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein.
It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions.
This gene is located on the long arm of chromosome 13.
Two splice variants encoding different isoforms have been found for this gene.
Applications:Suitable for use in ELISA.
Other applications have not been tested.
Recommended Dilutions: Peptide ELISA Titer: 1:8000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes RENT3A. Species sequence homology: human and bovine.This antibody is expected to recognize both isoforms (NP_075387.1; NP_542418.1).
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-KETDKQKKIAEKE, from the internal region of human RENT3A (NP_075387.1; NP_542418.1).
