Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry Other applications have not been tested.
Recommended Dilutions: Peptide ELISA Titer: 1:16,000Western Blot: 0.01-0.03ug/ml ~22kD band observed in lysates of cell lines HEK293, A549 and HepG2 (calculated MW of 21.1kD according to NP_002558.1).
Reported ~22kD in Rat Hippocampus
Immunohistochemistry: 1-2ug/ml In paraffin embedded Human Brain (Cortex) shows staining near sectect nuclei
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by peptide affinity chromatography.
Form:Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:Recognizes human PEBP1. Species sequence homology: mouse, rat, canine, bovine.
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide corresponding to C-DYVPKLYEQLSGK, from the C Terminus of human PEBP1 (NP_002558.1).