Applications:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilutions: Peptide ELISA Titer: 1:4000Western Blot: 0.03-1ug/ml ~26kD band observed in Human Kidney, Liver and Testis lysates (calculated MW of 21.7kD according to NP_006658.1).
In transfected HEK293 transiently expressing Human PGRMC1 a band of ~26kD is observed.
This band is not observed in the non-transfected HEK293.
The endogenous 20kD protein is observed in both
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by peptide affinity chromatography.
Form:Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:Recognizes human PGRMC1. Species sequence homology: mouse, rat.
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide corresponding to C-EPKDESARKND, from the C Terminus of human PGRMC1 (NP_006658.1).