Noxa is a mitochondrial localised protein and member of the BH3-only proapoptotic protein family, which is up-regulated by p53, IFNbeta, phorbol esters, dsRNA and viruses, and acts as promoter of caspase and apoptosis activation.
Noxa is a transcriptional target of p53, p73 and BH3-only proteins, and competes with, and displaces, the proapoptotic proteins BAK1, and BIM, from the antiapoptotioc protein MCL1 (Bcl-2-related protein EAT/mcl1), thereby influencing apoptosis versus cell survival.
Applications:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilutions: Peptide ELISA Titer: 1:16,000Western Blot: 0.03-0.1ug/ml detects ~16kD band in Human Brain (Cerebellum) lysates (calculated MW of 6.03kD according to NP_066950.1)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human NOXA.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-TQLRRFGDKLNFRQK, from the internal region of human NOXA (NP_066950.1).
