This gene encodes a member of the interferon regulatory transcription factor (IRF) family.
Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain.
Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome.
This protein is involved in palate formation.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions: Peptide ELISA Titer: 1:1:32,000Immunohistochemistry: 2-3ug/ml in paraffin embedded Human Kidney shows strong nuclear staining in DCT and glomerulusWestern Blot: 0.2-0.6ug/ml; ~60kD band observed in Human Ovary lysates (calculated MW of 53.1kD according to NP_006138.1).
In transfected HEK293 transiently expressing IRF6 a band of approx.
55kD is observed.
This band is not observed in the non-transfected HEK293Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human IRF6. Species sequence homology: mouse.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-TPSMQLPPALPPQ, from the C Terminus of human IRF6 (NP_006138.1).
