Applications:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilutions: Peptide ELISA Titer: 1:128,000Western Blot: 0.01-0.1ug/ml detects ~40kD band observed in Human Placenta lysates (calculated MW of 42.3kD according to NP_000853.1).
In transfected HEK293 transiently expressing Human HSD3B1 a band of approx.
45kD is observed.
This band is not observed in the non-transfected HEK293
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:Recognizes human HSD3B1.
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide corresponding to C-DRHKETLKSKTQ, from the C-terminus of human HSD3B1 (NP_000853.1).