This gene encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin.
Mutations in this gene have been associated with tuberous sclerosis.
Alternative splicing results in multiple transcript variants.
Applications:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilutions: Peptide ELISA Titer: 1:128,000Western Blot: 0.1-0.3ug/ml detects ~150kD band observed in Human Brain lysates (calculated MW of 130kD according to NP_000359.1).
The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miloloza et al Hum Mol Genet.
2000 Jul 22;9(12):1721-7; PMID: 10915759).
In transfected HEK293 transiently expressing Human TSC1 a band of approx.
120kD is observed.
This band is not observed in the non-transfected HEK293
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human Hamartin. This antibody is expected to recognise tuberous sclerosis isoform 1 (NP_000359.1) only. Species sequence homology: mouse, rat, canine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-QLHIMDYNETHHEH, from the C Terminus of human Hamartin (NP_000359.1).
