Bone morphogenetic proteins (e.g., BMP9; MIM 605120) are members of the transforming growth factor-beta (see TGFB1; MIM 190180) superfamily and regulate tissue differentiation and maintenance.
They are synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein.
Applications:Suitable for use in Peptide ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilutions: Peptide ELISA: 1:2000Western Blot: 0.1-0.3ug/ml detects ~35kD band observed in human prostate lysates (calculated MW of 34.1kD according to NP_004855.1).
In transfected HEK293 transiently expressing GDF15 a band of ~35kD is observed.
This band is not observed in the non-transfected HEK293
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human GDF15.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-QKTDTGVSLQTYDD from the C-terminus of human GDF15 (NP_004855.2) Species Sequence Homology: mouse, rat
