Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction.
FLOT1 encodes a caveolae-associated, integral membrane protein.
The function of flotillin 1 has not been determined.
Applications:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilutions: Peptide ELISA Titer: 1:32,000Western Blot: 0.3-1ug/ml ~48kD band observed in lysates of cell line H460 (calculated MW of 47.4kD according to NP_005794.1).
In transfected HEK293 transiently expressing FLOT1 a band of approx.
48kD is observed.
This band is not observed in the non-transfected HEK293
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human Flotillin 1. Species sequence homology: canine, porcine, bovine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-SISQVNHKPLRTA, from the C Terminus of human Flotillin 1 (NP_005794.1).
