F-box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box.
Some F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins.
In fact, F-box proteins are one of the four subunits of ubiquitin protein ligases, called SCFs.
SCF ligases bring ubiquitin conjugating enzymes to substrates that are specifically recruited by the different F-box proteins.
Mammalian F-box proteins are classified into three groups based on the presence of either WD-40 repeats, leucine-rich repeats, or the presence or absence of other protein-protein interacting domains.
This gene encodes the second identified member of the F-box gene family and contains multiple WD-40 repeats.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilutions: Peptide ELISA Titer: 1:128,000Western Blot: 0.3-1.5ug/ml; ~48kD band observed in Human Kidney lysates (calculated MW of 48.0kD according to NP_036296.1).
A stronger band of unknown identity was also consistently observed at appr.
30kD - both bands were successfully blocked by incubation with the immunising peptide
Immunohistochemistry: 2-3ug/ml In paraffin embedded Human Kidney, shows cytoplasmic staining of epithelial cells in some renal tubules
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human FBXW2. Species sequence homology: mouse.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-KRGSSFLAGEHPG, from the C Terminus of human FBXW2 (NP_036296.1).
