Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors.
This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases.
The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis.
Its kinase activity is stimulated by clathrin.
Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Applications:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilutions: Peptide ELISA Titer: 1:16,000Western Blot: 1-3mg/ml detecs an ~100kD band observed in Human Brain (Amygdala and Cerebellum) lysates (calculated MW of 104kD according to NP_055726.3)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human AAK1. Species sequence homology: mouse, rat, bovine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-GSPRTSQQNVYNPSE, from the internal region of human AAK1 (NP_055726.3),
