84-1162-83　［取扱停止］Matrix Metalloproteinase 14, Catalytic Domain, active (MT1-MMP, MMP-14, Collagenase-3) 100ug　M2429-05

特徴

• Matrix metalloproteinases (MMPs) are a family of secreted and membrane-bound zinc endopeptidases.
• Collectively, these enzymes degrade components of extracellular matrix (ECM), including collagens, fibronectin, laminin and other glycoproteins.
• MMPs play an important role in wound healing, apoptosis, development, angiogenesis, cancer metastases, and tissue remodeling.
• Most MMPs are secreted as inactive proproteins which are cleaved and activated; however, MT1-MMP (MMP-14) is a member of the membrane-type subfamily.
• Each member of this subfamily has a transmembrane domain suggesting these proteins are expressed at the cell’s surface rather than secreted.
• MT1-MMP is capable of mediating pericellular proteolysis of ECM components and thought to be an important tool for cellular remodeling of the ECM.
• It is also involved in endothelial cell migration, and although not fully characterized, MT1-MMP activity appears to modulate endothelial invasion, and formation of capillary tubes during angiogenesis (Galvez, 2001).
• It also appears to be a factor in monocyte recruitment during inflammation (Matias-Roman, 2005).
• MT1-MMP also activates MMP-2 protein, an activity involved in tumor invasion.
• Applications: Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry: 1:500Immunoprecipitation: 10ugOptimal dilutions to be determined by the researcher.
• Positive Control:HT-1080 cell lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.