Applications: Suitable for use in Western Blot and Immunohistochemistry
Other applications not tested.
Recommended Dilutions:Western Blot: 0.05ug/ml detected EOMES in 10ug of E13-14 mouse brain lysates
Immunohistochemistry (Paraffin): 1:100 from a previous lot detected EOMES in mouse and human brain.
Requires HIER with citrate buffer, pH 6.0Immunohistochemistry (Paraffin): 1:1000 from a representative lot detected EOMES in mouse cerebral cortex and mouse cerebellum tissue.
Requires HIER with citrate buffer, pH 6.0Optimal dilutions to be determined by the researcher.
Positive Control:E13-14 mouse brain lysate
Recommended Secondary Antibodies:I1906-10: IgY, Chicken (HRP) Rb x Ch I1906-12: IgY, Chicken (FITC) Rb x Ch I1906-14: IgY, Chicken (AP) Rb x Ch
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：chicken
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes mouse Tbr2 observed at ~85/95kD. Target bands appear larger than the calculated molecular size of 74.8kD (isoform 1) and 72.7kD (isoform 2). Species Crossreactivity: human and rat
Isotype：IgY
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Linear peptide corresponding to mouse EOMES, conjugated to KLH. Epitope: C-Terminus
