Human vimentin is a 53,521kD protein (466aa) expressed in many cell types of mesodermal origin, and is one of the intermediate filament types that form the cytoskeleton.
In the embryonic nervous system, however, vimentin is expressed by migrating neural crest cells forming the PNS, and by neural stem cells of the CNS.
In some forms of glioblastoma, vimenin is reexpressed GAP-43 by the tumor cells and serves as a general indicator of their undifferentiated state.
Applications:Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilutions: Immunohistochemistry: 1:1000-1:2000 using 2% paraformaldehyde tissuesImmunocytochemistry: 1:1000-1:2000 using 2% paraformaldehyde cellsWestern Blot: 1:2000-1:5000Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1906IgY, Chicken Pab Rb x Ch I1906-10IgY, Chicken (HRP) Rb x Ch I1906-12IgY, Chicken (FITC) Rb x Ch I1906-14IgY, Chicken (AP) Rb x Ch
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：300ug
Host：chicken
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes Vimentin. Species crossreactivity: human, mouse.
Isotype：IgY
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Three synthetic peptides corresponding to different regions of the Vimentin gene product (KLH). Species sequence homology: human (NP_003371, NCBI) and mouse (NP_035831, NCBI).
