Human TYH (EC 1.14.16.2) is a 58.523 kD protein (528aa) responsible for the enzymatic conversion of L-tyrosine to L-DOPA (dihydroxyphenylalanine).
This enzyme is expressed in all catecholaminergic neurons of the CNS and PNS.
In the CNS, TYH-positive neurons can be found within the substantia nigra, ventral tegmental area, locus ceruleus, and hypothalamus.
In the PNS, TYH-positive neurons can be found within the sympathetic chain, pre-vertebral ganglia and the adrenal medulla.
Applications:Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilutions: Immunohistochemistry: 1:1000-1:2000 using 2% paraformaldehyde tissuesImmunocytochemistry: 1:1000-1:2000 using 2% paraformaldehyde cellsWestern Blot: 1:2000-1:5000Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1906IgY, Chicken Pab Rb x Ch I1906-10IgY, Chicken (HRP) Rb x Ch I1906-12IgY, Chicken (FITC) Rb x Ch I1906-14IgY, Chicken (AP) Rb x Ch
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：chicken
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes Tyrosine Hydroxylase. Species crossreactivity: mouse. Species sequence homology: human, mouse.
Isotype：IgY
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Two synthetic peptides corresponding to Tyrosine Hydroxylase gene product (KLH). Species sequence homology: human (P07101, NCBI) and mouse (P24529, NCBI)
