Human Synaptotagmin-1 is a 47.442kD protein (422aa) associated with pre-synaptic terminals of the PNS and CNS.
Synaptotagmin-1 binds calcium in synaptic terminals and initiates the fusion of vesicles with the pre-synaptic membrane.
This protein is also believed to be the target of proteolytic activity associated with Botulinum neurotoxin A.
Applications:Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilutions: Immunohistochemistry: 1:1000-1:2000 using 2% paraformaldehyde tissuesImmunocytochemistry: 1:1000-1:2000 using 2% paraformaldehyde cellsWestern Blot: 1:2000-1:5000Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1906IgY, Chicken Pab Rb x Ch I1906-10IgY, Chicken (HRP) Rb x Ch I1906-12IgY, Chicken (FITC) Rb x Ch I1906-14IgY, Chicken (AP) Rb x Ch
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：400ug
Host：chicken
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes Synaptotagmin-1. Species sequence homology: human, mouse.
Isotype：IgY
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Four synthetic peptides corresponding to different regions of the Synaptotagmin-1 gene product (KLH). Species sequence homology: human (NP_005630, NCBI) and mouse (NP_033332, NCBI)
