Human PLP (also known as lipophilin) is a 29.946 kD protein (277 aa) found in myelin of the CNS and PNS.
Expressed by oligodendrocytes and Schwann cells, PLP stabilizes myelin by preventing lipid bilayer fusion, and aids in its compaction.
Different mutations of the human PLP gene product result in two neurological disorders -- Pelizaeus-Merzbacher disease and Spastic Paraplegia type 2.
Applications:Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilutions: Immunohistochemistry: 1:1000-1:2000 using 2% paraformaldehyde tissuesImmunocytochemistry: 1:1000-1:2000 using 2% paraformaldehyde cellsWestern Blot: 1:2000-1:5000Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1906IgY, Chicken Pab Rb x Ch I1906-10IgY, Chicken (HRP) Rb x Ch I1906-12IgY, Chicken (FITC) Rb x Ch I1906-14IgY, Chicken (AP) Rb x Ch
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：chicken
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes Proteolipid Protein. Species crossreactivity: mouse. Species sequence homology: human, mouse.
Isotype：IgY
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to a region of the Proteolipid Protein gene product (KLH). Species sequence homology: human (NP_000524, PLP1 isoform 1 and PLP1 isoform 2 (NP_955772)] and mouse (P60202).
