Human Perpherin is a 53.651kD (470 aa) expressed in all PNS neurons and some CNS neurons, including a subset of cortical neurons, hippocampal neurons, and ventral horn spinal cord motor neurons.
At an ultrastructural level, peripherin immunoreactivity is associated with the neurofilament portion of the cytoskeleton, being particularly abundant in the cell body.
Peripherin protein has been shown to undergo upregulation during periods of trophic stress in cultured neurons, and mutations of the peripherin gene have been associated with the neurodegenerative disease amyotrophic lateral sclerosis.
Applications:Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilutions: Immunohistochemistry: 1:2000-1:5000 using 2% paraformaldehyde tissuesImmunocytochemistry: 1:2000-1:5000 using 2% paraformaldehyde cellsWestern Blot: 1:5000-1:10,000Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1906IgY, Chicken Pab Rb x Ch I1906-10IgY, Chicken (HRP) Rb x Ch I1906-12IgY, Chicken (FITC) Rb x Ch I1906-14IgY, Chicken (AP) Rb x Ch
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：chicken
Source Antibody：mouse
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% sodium azide.
Specificity：Recognizes human Peropherin.
Isotype：IgY
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Full-length recombinant human peripherin protein.
