Human NSE-2 (EC 4.2.1.11) is a 47.138 kD protein (434aa) expressed in neurons of the peripheral nervous system (PNS) and central nervous system (CNS).
NSE-2 catalyzes the conversion of 2-phospho-D-glycerate into phosphoenol pyruvate, and is an essential enzyme in energy metabolism in nervous tissues.
Applications:Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilutions: Immunohistochemistry: 1:2000-1:5000 using 2% paraformaldehyde tissuesImmunocytochemistry: 1:2000-1:5000 using 2% paraformaldehyde cellsWestern Blot: 1:5000-1:10,000Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1906:IgY, Chicken Pab Rb x Ch I1906-10:IgY, Chicken (HRP) Rb x Ch I1906-12:IgY, Chicken (FITC) Rb x Ch I1906-14:IgY, Chicken (AP) Rb x Ch
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：chicken
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes Neuron-Specific Enolase type 2. Species Crossreactivity: mouse
Isotype：IgY
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Two synthetic peptides corresponding to different regions of the Neuron-Specific Enolase, type-2 gene product (KLH). Species sequence homology: human (NP_001966) and rat (AAA41119)
