Murine netrin-1 precursor is a 67.768 Kd protein (604aa) expressed by subpopulations of cells within the developing CNS, as well as in various non- neural tissues, including the gut, liver, heart, and prostate.
In the embryonic CNS, netrin-1 is expressed by cells of the floor plate, and acts to attract axons of commissural neurons from the dorsal horn gray matter.
Netrin-1 is also believed to play a role in the growth of motor axons in the PNS.
Applications:Suitable for use in Immunohistochemistry and Immunocytochemistry.
Other applications have not been tested.
Recommended Dilutions: Immunohistochemistry: 1:1000-1:2000 using 2% paraformaldehyde tissuesImmunocytochemistry: 1:1000-1:2000 using 2% paraformaldehyde cellsOptimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1906IgY, Chicken Pab Rb x Ch I1906-10IgY, Chicken (HRP) Rb x Ch I1906-12IgY, Chicken (FITC) Rb x Ch I1906-14IgY, Chicken (AP) Rb x Ch
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：chicken
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes Netrin-1. Species crossreactivity: mouse. Species sequence homology: human.
Isotype：IgY
Calc Applications Abbrev：IC IHC
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to Netrin-1 (KLH). Species sequence homology: human (O_095631, NCBI) and mouse (O_09118, NCBI)
