Human myelin basic protein (MBP) is a 20.115 Kd protein (186aa) found in myelin of the CNS and PNS.
Expressed as an intracellular protein by oligodendrocytes and Schwann cells, MBP aids in the compaction and stability of myelin.
Applications:Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilutions: Immunohistochemistry: 1:1000-1:2000 using 2% paraformaldehyde tissuesImmunocytochemistry: 1:1000-1:2000 using 2% paraformaldehyde cellsWestern Blot: 1:2000-1:5000Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1906IgY, Chicken Pab Rb x Ch I1906-10IgY, Chicken (HRP) Rb x Ch I1906-12IgY, Chicken (FITC) Rb x Ch I1906-14IgY, Chicken (AP) Rb x Ch
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:chicken
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity:Recognizes Myelin Basic Protein. Species crossreactivity: mouse. Species sequence homology: human and mouse.
Isotype:IgY
Calc Applications Abbrev:IC IHC WB
Calc Crossreactivity:Mo
Immunogen:Synthetic peptide corresponding to Myelin Basic Protein (KLH). Species sequence homology: human (NP_002376, NCBI) and mouse (NP_034907, NCBI).